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Home > Product > ELISA Kit > Human 26S Protease Regulatory Subunit 6B, PSMC4 ELISA Kit

Human 26S Protease Regulatory Subunit 6B, PSMC4 ELISA Kit

Human 26S Protease Regulatory Subunit 6B, PSMC4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.45ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.45ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

26S Protease Regulatory Subunit 6B

Code

E4873Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.45ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC4 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into Peptides.

UniProt accession

MASS(Da)

47,366

GeneID

5704

Synonyms

26S proteasome AAA-ATPase subunit RPT3;26S proteasome regulatory subunit 6B;MB67-interacting protein;MIP224;Proteasome 26S subunit ATPase 4;PSMC 4;PSMC4;Tat-binding protein 7;TBP-7;

Gene names

PSMC4

Research area

Others

Target protein

PSMC4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4873Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.14 2.277 2.22
2.414
800ng/L 1.405 1.371 1.314
1.337
400ng/L 0.82 0.754 0.697
0.689
200ng/L 0.545 0.518 0.462
0.491
100ng/L 0.32 0.281 0.225
0.243
0ng/L 0.048 0.057 0
0.066

Precision

We measured random samples of E4873Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 152.1 11.06 7.3
Intra-Assay 2 18 793.5 24.85 3.1
Intra-Assay 3 18 418.1 25.7 6.1

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E4873Hu

    $320.00

  • 96T

    E4873Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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