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Home > Product > ELISA Kit > Canine Orexin, HCRT ELISA Kit

Canine Orexin, HCRT ELISA Kit

Canine Orexin, HCRT ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.27ng/L

Detection range: 0.5-200ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product Summary

Size: 96T,48T

Sensitivity: 0.27ng/L

Detection range: 0.5-200ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product overview

Full product name

Orexin

Code

E0365Ca

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.27ng/L

Detection range

0.5-200ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Canine

Storage

2-8ºC

Assay time

1h 30m

Background

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.

UniProt accession

MASS(Da)

13,328

GeneID

607641

Synonyms

HCRT;Hcrt1;Hcrt2;Hypocretin;Hypocretin-1;Hypocretin-2;Orexin;Orexin-A;Orexin-B;

Gene names

HCRT

Research area

Metabolism

Target protein

HCRT

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0365Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
120ng/L 2.293 2.283 2.247
2.273
60ng/L 1.372 1.304 1.268
1.236
30ng/L 0.624 0.612 0.576
0.6
15ng/L 0.404 0.389 0.353
0.373
7.5ng/L 0.179 0.191 0.155
0.202
0ng/L 0.053 0.036 0
0.019

Precision

We measured random samples of E0365Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 18.2 1.1 6
Intra-Assay 2 18 33.1 1.36 4.1
Intra-Assay 3 18 86.7 2.56 3

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 96T

    E0365Ca

    $475.00

  • 48T

    E0365Ca

    $350.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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