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Home > Product > ELISA Kit > Human Myeloid Cell Surface Antigen Cd33, CD33 ELISA Kit

Human Myeloid Cell Surface Antigen Cd33, CD33 ELISA Kit

Human Myeloid Cell Surface Antigen Cd33, CD33 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 23.55ng/L

Detection range: 70-15000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 23.55ng/L

Detection range: 70-15000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Myeloid Cell Surface Antigen CD33

Code

E5714Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

23.55ng/L

Detection range

70-15000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed: 10611343, PubMed: 15597323, PubMed: 11320212). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed: 7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed: 28325905, PubMed: 10887109). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed: 10556798, PubMed: 10206955, PubMed: 10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed: 10206955, PubMed: 10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed: 15597323).

UniProt accession

MASS(Da)

39,825

GeneID

945

Synonyms

CD 33;CD antigen CD33;CD33;gp67;Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3

Gene names

CD33

Research area

Immunology

Target protein

CD33

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5714Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8000ng/L 2.264 2.15 2.102
2.035
4000ng/L 1.948 1.83 1.781
1.711
2000ng/L 1.259 1.277 1.229
1.296
1000ng/L 0.746 0.697 0.649
0.649
500ng/L 0.349 0.387 0.338
0.424
0ng/L 0.044 0.048 0
0.052

Precision

We measured random samples of E5714Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 498.1 20.68 4.2
Intra-Assay 2 18 2337.5 93.59 4
Intra-Assay 3 18 1714.9 116.57 6.8

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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