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Home > Product > ELISA Kit > Human Insulin, INS ELISA Kit

Human Insulin, INS ELISA Kit

Human Insulin, INS ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.11mIU/L

Detection range: 0.2-60mIU/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.11mIU/L

Detection range: 0.2-60mIU/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Insulin

Code

E0010Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.11mIU/L

Detection range

0.2-60mIU/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

UniProt accession

MASS(Da)

11981

GeneID

3630

Synonyms

INS;Insulin A chain;Insulin B chain

Gene names

INS

Research area

Metabolism

Target protein

INS

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0010Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40mIU/L 2.28 2.194 2.152
2.109
20mIU/L 1.174 1.286 1.244
1.398
10mIU/L 0.839 0.741 0.7
0.644
5mIU/L 0.399 0.438 0.396
0.477
2.5mIU/L 0.235 0.212 0.17
0.188
0mIU/L 0.026 0.042 0
0.058

Precision

We measured random samples of E0010Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.8 0.52 5.9
Intra-Assay 2 18 30.5 1.19 3.9
Intra-Assay 3 18 13.3 0.7 5.2

Protocols

References (8)

Calcium and vitamin D3 combinations improve fatty liver disease through AMPK-independent mechanisms
Bitter gourd reduces elevated fasting plasma glucose levels in an intervention study among prediabetics in Tanzania
Asprosin in umbilical cord of newborns and maternal blood of gestational diabetes, preeclampsia, severe preeclampsia, intrauterine growth retardation and macrosemic fetus
Dietary energy density, metabolic parameters, and blood pressure in a sample of adults with obesity
Evaluating the association between the Mediterranean-DASH Intervention for Neurodegenerative Delay (MIND) diet, mental health, and cardio-metabolic risk factors among individuals with obesity
View all publications for the product

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  • Size

    Code

    Price

  • 96T

    E0010Hu

    $458.00

  • 48T

    E0010Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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