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Mouse Interleukin 6, IL-6 ELISA Kit

Mouse Interleukin 6, IL-6 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.51pg/ml

Detection range: 3-900pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.51pg/ml

Detection range: 3-900pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Interleukin 6

Code

E0049Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.51pg/ml

Detection range

3-900pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism (Probable). Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (PubMed: 8910279). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (PubMed: 27893700). IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B-cells into immunoglolin-secreting cells (Probable). Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Together with IL21, controls the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (PubMed: 23045607). Required to drive naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells (PubMed: 16990136, PubMed: 27893700). Also required for proliferation of myeloma cells and the survival of plasmablast cells (Probable). Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF (PubMed: 17075861). Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance (PubMed: 11786910). 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis (PubMed: 28402851). Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand (PubMed: 11113088). Also acts as a myokine (By similarity). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (PubMed: 11113088, PubMed: 8910279). Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection (PubMed: 15124018). Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity).

UniProt accession

MASS(Da)

24,384

GeneID

16193

Synonyms

B-cell hybridoma growth factor;IL 6;IL6;IL-6;Interleukin HP-1;Interleukin-6

Gene names

IL6

Research area

Immunology; Neuroscience; Microbiology; Cancer; Metabolism

Target protein

IL6

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Mouse Interleukin 6, IL-6 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480pg/ml 2.503 2.439 2.387
2.374
240pg/ml 1.358 1.378 1.326
1.397
120pg/ml 0.946 0.87 0.819
0.794
60pg/ml 0.403 0.468 0.416
0.533
30pg/ml 0.188 0.229 0.177
0.269
0pg/ml 0.04 0.051 0
0.063

Precision

We measured random samples of E0049Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 132.4 7.48 5.6
Intra-Assay 2 18 309.4 9.79 3.2
Intra-Assay 3 18 53.5 3.37 6.3

Protocols

References

PENGARUH PEMBERIAN KRIM TOPIKAL EKSTRAK BUAH NAGA MERAH (Hylocereus polyrhizuz) PADA LUKA AKUT TERHADAP KADAR INTERLEUKIN-6 FASE INFLAMASI
Modulating effects of the synergistic combination of extracts of herbal drugs on cyclophosphamide-induced immunosuppressed mice
Modulating effects of the synergistic combination of extracts of herbal drugs on cyclophosphamide-induced immunosuppressed mice

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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