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Home > Product > ELISA Kit > Mouse Tumor Necrosis Factor Αlpha, TNF-A ELISA Kit

Mouse Tumor Necrosis Factor Αlpha, TNF-A ELISA Kit

Mouse Tumor Necrosis Factor Αlpha, TNF-A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.49ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 2.49ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Tumor Necrosis Factor alpha

Code

E0117Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.49ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed: 25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

UniProt accession

MASS(Da)

25,896

GeneID

21926

Synonyms

Cachectin;C-domain 1;C-domain 2;ICD1;ICD2;Intracellular domain 1;Intracellular domain 2;N-terminal fragment;NTF;TNF A;TNF alpha;TNFA;TNF-a;TNF-alpha;Tumor necrosis factor;Tumor necrosis factor ligand superfamily member 2;Tumor necrosis factor, membrane form;Tumor necrosis factor, soluble form

Gene names

TNFA

Research area

Immunology; Signal Transduction; Cancer; Microbiology

Target protein

TNFA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Mouse Tumor Necrosis Factor Αlpha, TNF-A ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.342 2.349 2.314
2.355
320ng/L 1.414 1.437 1.402
1.46
160ng/L 0.826 0.777 0.742
0.727
80ng/L 0.481 0.483 0.448
0.486
40ng/L 0.273 0.277 0.242
0.28
0ng/L 0.036 0.035 0
0.034

Precision

We measured random samples of E0117Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 130.1 8.28 6.4
Intra-Assay 2 18 305.7 11.44 3.7
Intra-Assay 3 18 169.6 8.45 5

Protocols

References

Modulating effects of the synergistic combination of extracts of herbal drugs on cyclophosphamide-induced immunosuppressed mice
Modulating effects of the synergistic combination of extracts of herbal drugs on cyclophosphamide-induced immunosuppressed mice

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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