Mouse Peroxisome Proliferator-activated Receptor Gamma, PPAR-G ELISA Kit

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Home > Product > ELISA Kit > Mouse Peroxisome Proliferator-activated Receptor Gamma, PPAR-G ELISA Kit

Mouse Peroxisome Proliferator-activated Receptor Gamma, PPAR-G ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.26ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.26ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Peroxisome Proliferator-Activated Receptor Gamma

Code

E0316Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.26ng/ml

Detection range

0.5-100ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (PubMed: 19041764).

UniProt accession

P37238

MASS（Da）

57598

GeneID

19016

Synonyms

Nuclear receptor subfamily 1 group C member 3;Peroxisome proliferator-activated receptor gamma;PPARG;PPAR-gamma

Gene names

PPARG

Research area

Metabolism

Target protein

PPARG

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E0316Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.358	2.33	2.285
	2.302
32ng/ml	1.718	1.619	1.574
	1.519
16ng/ml	1.004	0.983	0.938
	0.962
8ng/ml	0.733	0.681	0.636
	0.63
4ng/ml	0.437	0.444	0.399
	0.451
0ng/ml	0.026	0.045	0
	0.064

Precision

We measured random samples of E0316Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	9.6	0.46	4.8
Intra-Assay	2	18	27.9	1.25	4.5
Intra-Assay	3	18	4.9	0.37	7.6

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E0316Mo

$320.00
96T

E0316Mo

$458.00

Add to Cart

Lead time: Within two weeks

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