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Home > Product > ELISA Kit > Mouse Protein O-Glucosyltransferase 1, POGLUT1 ELISA Kit

Mouse Protein O-Glucosyltransferase 1, POGLUT1 ELISA Kit

Mouse Protein O-Glucosyltransferase 1, POGLUT1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.151ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.151ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Protein O-Glucosyltransferase 1

Code

E0720Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.151ng/ml

Detection range

0.3-90ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Dual specificity glycosyltransferase that catalyzes the transfer of glucose and xylose from UDP-glucose and UDP-xylose, respectively, to a serine residue found in the consensus sequence of C-X-S-X-P-C (PubMed: 21949356, PubMed: 26496195). Specifically targets extracellular EGF repeats of protein such as CRB2, F7, F9 and NOTCH2 (PubMed: 21949356, PubMed: 26496195). Acts as a positive regulator of Notch signaling by mediating O-glucosylation of Notch, leading to regulate muscle development (By similarity). Notch glucosylation does not affect Notch ligand binding (By similarity). Required during early development to promote gastrulation: acts by mediating O-glucosylation of CRB2, which is required for CRB2 localization to the cell membrane (PubMed: 26496195).

UniProt accession

MASS(Da)

46,379

GeneID

224143

Synonyms

CAP10-like 46 kDa protein;KTEL motif-containing protein 1;O-glucosyltransferase Rumi homolog;POGLUT 1;POGLUT1;Protein O-glucosyltransferase 1;Protein O-xylosyltransferase POGLUT1;Rumi;Wing-shaped neural plate protein;Wsnp

Gene names

POGLUT1

Research area

Others

Target protein

POGLUT1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0720Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/ml 2.02 2.152 2.129
2.283
24ng/ml 1.544 1.481 1.458
1.418
12ng/ml 0.737 0.711 0.688
0.685
6ng/ml 0.425 0.424 0.401
0.423
3ng/ml 0.127 0.146 0.123
0.165
0ng/ml 0.024 0.023 0
0.023

Precision

We measured random samples of E0720Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.7 0.31 4.1
Intra-Assay 2 18 12.6 0.51 4
Intra-Assay 3 18 20.7 0.58 2.8

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E0720Mo

    $320.00

  • 96T

    E0720Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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