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Home > Product > ELISA Kit > Mouse Cytochrome P450 1A2, CYP1A2 ELISA Kit

Mouse Cytochrome P450 1A2, CYP1A2 ELISA Kit

Mouse Cytochrome P450 1A2, CYP1A2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.12ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.12ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Cytochrome P450 1A2

Code

E0819Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.12ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer. Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. Metabolizes caffeine via N3-demethylation.

UniProt accession

MASS(Da)

58,184

GeneID

13077

Synonyms

Cholesterol 25-hydroxylase;CYP1A2;CYPIA2;Cytochrome P450 1A2;Cytochrome P450-P2;Cytochrome P450-P3;Hydroperoxy icosatetraenoate dehydratase

Gene names

CYP1A2

Research area

Others

Target protein

CYP1A2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Mouse Cytochrome P450 1A2, CYP1A2 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.625 2.595 2.551
2.566
20ng/ml 1.463 1.436 1.392
1.41
10ng/ml 0.869 0.9 0.856
0.932
5ng/ml 0.453 0.476 0.432
0.499
2.5ng/ml 0.233 0.25 0.205
0.266
0ng/ml 0.03 0.044 0
0.058

Precision

We measured random samples of E0819Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 13.5 0.71 5.3
Intra-Assay 2 18 24.3 0.7 2.9
Intra-Assay 3 18 7.3 0.54 7.4

Protocols

References

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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