Tel:86 021 3100 7137

2022 Holiday shipping schedule: International shipments will proceed through Friday 21st January, click to know more
Home > Product > ELISA Kit > Mouse Delta Like Protein 1, DLL1 ELISA Kit

Mouse Delta Like Protein 1, DLL1 ELISA Kit

Mouse Delta Like Protein 1, DLL1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 34.57ng/L

Detection range: 70-15000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 34.57ng/L

Detection range: 70-15000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Delta Like Protein 1

Code

E1391Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

34.57ng/L

Detection range

70-15000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Transmembrane ligand protein of NOTCH1, NOTCH2 and NOTCH3 receptors that binds the extracellular domain (ECD) of Notch receptor in a cis and trans fashion manner (PubMed: 21985982, PubMed: 10958687). Following transinteraction, ligand cells produce mechanical force that depends of a clathrin-mediated endocytosis, requiring ligand ubiquitination, EPN1 interaction, and actin polymerisation; these events promote Notch receptor extracellular domain (NECD) transendocytosis and triggers Notch signaling through induction of cleavage, hyperphosphorylation, and nuclear accumulation of the intracellular domain of Notch receptors (NICD) (PubMed: 10958687, PubMed: 18676613). Is required for embryonic development and maintenance of adult stem cells in many different tissues and immune systeme; the DLL1-induced Notch signaling is mediated through an intercellular communication that regulates cell lineage, cell specification, cell patterning and morphogenesis through effects on differentiation and proliferation (PubMed: 1719$475.00|$350.009, PubMed: 19562077, PubMed: 18997111, PubMed: 23695674, PubMed: 16495313, PubMed: 21238454, PubMed: 22282195, PubMed: 7671806, PubMed: 17960184, PubMed: 22529374, PubMed: 19389377, PubMed: 23699523, PubMed: 1914$498.00|$360.009, PubMed: 23688253, PubMed: 23806616, PubMed: 26114479, PubMed: 22940113, PubMed: 25220152, PubMed: 20081190, PubMed: 21572390, PubMed: 22096075). Plays a role in brain development at different level, namely by regulating neuronal differentiation of neural precursor cells via cell-cell interaction, most likely through the lateral inhibitory system in an endogenous level dependent-manner (PubMed: 7671806, PubMed: 18997111). During neocortex development, Dll1-Notch signaling transmission is mediated by dynamic interactions between intermediate neurogenic progenitors and radial glia; the cell-cell interactions are mediated via dynamic and transient elongation processes, likely to reactivate/maintain Notch activity in neighboring progenitors, and coordinate progenitor cell division and differentiation across radial and zonal boundaries (PubMed: 23699523). During cerebellar development, regulates Bergmann glial monolayer formation and its morphological maturation through a Notch signaling pathway (PubMed: 23688253). At the retina and spinal cord level, regulates neurogenesis by preventing the premature differentiation of neural progenitors and also by maintaining progenitors in spinal cord through Notch signaling pathway (PubMed: 19389377, PubMed: 26114479). Also controls neurogenesis of the neural tube in a progenitor domain-specific fashion along the dorsoventral axis (PubMed: 20081190). Maintains quiescence of neural stem cells and plays a role as a fate determinant that segregates asymmetrically to one daughter cell during neural stem cells mitosis, resulting in neuronal differentiation in Dll1-inheriting cell (PubMed: 23695674). Plays a role in immune systeme development, namely the development of all T-cells and marginal zone (MZ) B cells (PubMed: 15146182, PubMed: 19217325). Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor (By similarity). Upon MMP14 cleavage, negatively regulates Notch signaling in haematopoietic progenitor cells to specifically maintain normal B-cell development in bone marrow (PubMed: 21572390). Also plays a role during muscle development. During early development, inhibits myoblasts differentiation from the medial dermomyotomal lip and later regulates progenitor cell differentiation (PubMed: 1719$475.00|$350.009). Directly modulates cell adhesion and basal lamina formation in satellite cells through Notch signaling. Maintains myogenic progenitors pool by suppressing differentiation through down-regulation of MYOD1 and is required for satellite cell homing and PAX7 expression (PubMed: 22940113). During craniofacial and trunk myogenesis suppresses differentiation of cranial mesoderm-derived and somite-derived muscle via MYOD1 regulation but in cranial mesoderm-derived progenitors, is neither required for satellite cell homing nor for PAX7 expression (PubMed: 25220152). Also plays a role during pancreatic cell development. During type B pancreatic cell development, may be involved in the initiation of proximodistal patterning in the early pancreatic epithelium (PubMed: 22529374). Stimulates multipotent pancreatic progenitor cells proliferation and pancreatic growth by maintaining HES1 expression and PTF1A protein levels (PubMed: 22096075). During fetal stages of development, is required to maintain arterial identity and the responsiveness of arterial endothelial cells for VEGFA through regulation of KDR activation and NRP1 expression (PubMed: 1914$498.00|$360.009). Controls sprouting angiogenesis and subsequent vertical branch formation througth regulation on tip cell differentiation (PubMed: 22282195). Negatively regulates goblet cell differentiation in intestine and controls secretory fat commitment through lateral inhibition in small intestine (PubMed: 21238454, PubMed: 21915337). Plays a role during inner ear development; negatively regulates auditory hair cell differentiation (PubMed: 16495313). Plays a role during nephron development through Notch signaling pathway (PubMed: 23806616). Regulates growth, blood pressure and energy homeostasis (PubMed: 19562077).

UniProt accession

MASS(Da)

78,449

GeneID

13388

Synonyms

Delta1;Delta-like protein 1;DLL 1;DLL1;Dll1-derived cell-associated form;Dll1-EC;Dll1-IC;Dll1-intracellular form;Dll1-soluble form;Dll1-TMIC;Drosophila Delta homolog 1;Membrane-associated fragment;Shed form;

Gene names

DLL1

Research area

Others

Target protein

DLL1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Mouse Delta Like Protein 1, DLL1 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8000ng/L 1.822 1.873 1.828
1.923
4000ng/L 1.069 0.999 0.954
0.929
2000ng/L 0.522 0.504 0.459
0.485
1000ng/L 0.288 0.266 0.221
0.244
500ng/L 0.16 0.164 0.119
0.169
0ng/L 0.06 0.045 0
0.03

Precision

We measured random samples of E1391Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2850.1 194.43 6.8
Intra-Assay 2 18 5677.8 132.37 2.3
Intra-Assay 3 18 2974.5 144.41 4.9

Protocols

References

Customer reviews and Q&As

No reviews
No reviews

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up