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Home > Product > ELISA Kit > Mouse Neprilysin, NEP ELISA Kit

Mouse Neprilysin, NEP ELISA Kit

Mouse Neprilysin, NEP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Neprilysin

Code

E1781Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.026ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid Peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (By similarity). Involved in the degradation of the atrial natriuretic factor (ANF) (By similarity). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed: 20876573).

UniProt accession

MASS(Da)

85,702

GeneID

17380

Synonyms

Atriopeptidase;CD antigen CD10;Enkephalinase;MME;NEP;Neprilysin;Neutral endopeptidase;Neutral endopeptidase 24.11;SFE;Skin fibroblast elastase

Gene names

MME

Research area

Others

Target protein

MME

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1781Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.304 2.272 2.23
2.241
6ng/mL 1.516 1.498 1.455
1.479
3ng/mL 1.004 1.033 0.991
1.061
1.5ng/mL 0.754 0.736 0.694
0.718
0.75ng/mL 0.485 0.483 0.44
0.48
0ng/mL 0.028 0.042 0
0.056

Precision

We measured random samples of E1781Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.3 0.37 5.9
Intra-Assay 2 18 1.8 0.14 7.7
Intra-Assay 3 18 0.7 0.05 7.3

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1781Mo

    $320.00

  • 96T

    E1781Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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