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Home > Product > ELISA Kit > Mouse Angiogenin, ANG ELISA Kit

Mouse Angiogenin, ANG ELISA Kit

Mouse Angiogenin, ANG ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.78ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.78ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Angiogenin

Code

E1837Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.78ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo (By similarity).

UniProt accession

MASS(Da)

16,228

GeneID

11727

Synonyms

ANG;Angiogenin;Angiogenin-1;Ribonuclease 5;RNase 5

Gene names

ANG

Research area

Signal Transduction; Epigenetics and Nuclear Signaling; Neuroscience; Cardiovascular; Cancer

Target protein

ANG

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1837Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.148 2.064 2.032
1.98
1600ng/L 1.437 1.449 1.417
1.461
800ng/L 0.591 0.574 0.542
0.557
400ng/L 0.413 0.417 0.384
0.42
200ng/L 0.206 0.165 0.132
0.123
0ng/L 0.034 0.032 0
0.031

Precision

We measured random samples of E1837Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2170.2 122.05 5.6
Intra-Assay 2 18 1030.3 37.92 3.7
Intra-Assay 3 18 663.1 27.78 4.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E1837Mo

    $320.00

  • 96T

    E1837Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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