Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Mouse C-C Chemokine Receptor Type 2, CCR2 ELISA Kit

Mouse C-C Chemokine Receptor Type 2, CCR2 ELISA Kit

Mouse C-C Chemokine Receptor Type 2, CCR2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.49ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.49ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

C-C Chemokine Receptor Type 2

Code

E1916Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.49ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Key functional receptor for CCL2 but can also bind CCL7 and CCL12 chemokines (PubMed: 8631787, PubMed: 8662823, PubMed: 8996246). Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (By similarity). Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B (By similarity). Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation (PubMed: 28507030). Facilitates the export of mature thymocytes by enhancing directional movement of thymocytes to sphingosine-1-phosphate stimulation and up-regulation of S1P1R expression; signals through the JAK-STAT pathway to regulate FOXO1 activity leading to an increased expression of S1P1R (PubMed: 29930553). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed: 29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed: 29993042). Mediates the recruitment of macrophages and monocytes to the injury site following brain injury (PubMed: 24806994, PubMed: 29632244).

UniProt accession

MASS(Da)

42,783

GeneID

12772

Synonyms

C-C chemokine receptor type 2;C-C CKR-2;CC-CKR-2;CCR 2;CCR2;CCR-2;CD antigen CD192;JE/FIC receptor;MCP-1 receptor;

Gene names

CCR2

Research area

Immunology

Target protein

CCR2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1916Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.155 2.139 2.085
2.123
600ng/L 1.042 1.082 1.028
1.123
300ng/L 0.424 0.464 0.409
0.503
150ng/L 0.222 0.203 0.149
0.184
75ng/L 0.125 0.138 0.084
0.152
0ng/L 0.048 0.054 0
0.06

Precision

We measured random samples of E1916Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 684.5 27.89 4.1
Intra-Assay 2 18 914.2 25.88 2.8
Intra-Assay 3 18 257.1 10.62 4.1

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E1916Mo

    $320.00

  • 96T

    E1916Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up