Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway.
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E3311Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 16ng/ml | 2.516 | 2.473 | 2.374 |
| 2.43 | |||
| 8ng/ml | 1.511 | 1.551 | 1.452 |
| 1.591 | |||
| 4ng/ml | 0.993 | 1.017 | 0.918 |
| 1.041 | |||
| 2ng/ml | 0.896 | 0.936 | 0.837 |
| 0.976 | |||
| 1ng/ml | 0.634 | 0.597 | 0.498 |
| 0.561 | |||
| 0ng/ml | 0.103 | 0.099 | 0 |
| 0.094 |
Precision
We measured random samples of E3311Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 5.3 | 0.2 | 3.5 |
| Intra-Assay | 2 | 18 | 4.4 | 0.3 | 6.4 |
| Intra-Assay | 3 | 18 | 1 | 0 | 4.7 |
Protocols
References
Customer reviews and Q&As
