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Target protein

Components	Quantity
Pre-coated plate	8 wells * 12 detachable strips
The standard, lyophilized	2 vials
Standard/Sample diluent	6ml x 1 vial
Biotinylated antigen, lyophilized	1 vial
Avidin-HRP concentrate	100μl x 1 vial
Biotinylated antigen diluent	6ml x 1 vials
Avidin HRP diluent	5.9ml x 1 vials
Substrate solution A	6ml x1 vial
Substrate solution B	6ml x1 vial
Stop solution	6ml x1 vial
Wash buffer concentrate (25x)	20ml x 1 vial

These standard curves of EA0132Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
0nmol/L	2.347	2.273	2.22
	2.199
15nmol/L	1.833	1.903	1.85
	1.973
30nmol/L	1.152	1.18	1.127
	1.209
60nmol/L	0.719	0.754	0.701
	0.789
120nmol/L	0.475	0.445	0.392
	0.414
240nmol/L	0.147	0.142	0.089
	0.138

We measured random samples of EA0132Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	15.8	0.8	5
Intra-Assay	2	18	73.1	4.5	6.2
Intra-Assay	3	18	34.5	2.5	7.3