Product overview
Full product name
Size
Sensitivity
Detection range
Sample type
Code
Assay type
Species
Storage
Assay time
Synonyms
Research area
Target protein
Components
Components | Quantity |
---|---|
Pre-coated plate | 12 * 8 well strips x 1 |
The standard, lyophilized | 2 vials |
Standard/Sample diluent | 6ml x 1 vial |
Biotinylated antigen, lyophilized | 1 vial |
Avidin-HRP concentrate | 100μl x 1 vial |
Biotinylated antigen diluent | 6ml x 1 vials |
Avidin HRP diluent | 5.9ml x 1 vials |
Substrate solution A | 6ml x1 vial |
Substrate solution B | 6ml x1 vial |
Stop solution | 6ml x1 vial |
Wash buffer concentrate (25x) | 20ml x 1 vial |
Protocol data
These standard curves of EA0132Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
0nmol/L | 2.347 | 2.273 | 2.22 |
2.199 | |||
15nmol/L | 1.833 | 1.903 | 1.85 |
1.973 | |||
30nmol/L | 1.152 | 1.18 | 1.127 |
1.209 | |||
60nmol/L | 0.719 | 0.754 | 0.701 |
0.789 | |||
120nmol/L | 0.475 | 0.445 | 0.392 |
0.414 | |||
240nmol/L | 0.147 | 0.142 | 0.089 |
0.138 |
Precision
We measured random samples of EA0132Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-Assay | 1 | 18 | 15.8 | 0.8 | 5 |
Intra-Assay | 2 | 18 | 73.1 | 4.5 | 6.2 |
Intra-Assay | 3 | 18 | 34.5 | 2.5 | 7.3 |
Protocols
References
Customer reviews and Q&As
Associated products