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Home > Product > ELISA Kit > Mouse Clusterin, CLU ELISA Kit

Mouse Clusterin, CLU ELISA Kit

Mouse Clusterin, CLU ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.17ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.17ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Clusterin

Code

E1943Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.17ng/mL

Detection range

0.3-90ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins (By similarity). Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro) (PubMed: 14741101). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity (By similarity). Following stress, promotes apoptosis (PubMed: 12551933). Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation. Following ER stress, suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. When secreted, does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (By similarity). When secreted, acts as an important modulator during neuronal differentiation through interaction with STMN3 (By similarity). Plays a role in the clearance of immune complexes that arise during cell injury (PubMed: 11865066).

UniProt accession

MASS(Da)

51,656

GeneID

12759

Synonyms

Apo-J;Apolipoprotein J;CLU;Clusterin;Clusterin alpha chain;Clusterin beta chain;Clustrin;SGP-2;Sulfated glycoprotein 2;

Gene names

CLU

Research area

Cardiovascular

Target protein

CLU

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1943Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/mL 2.047 2.153 2.108
2.259
24ng/mL 1.764 1.805 1.76
1.847
12ng/mL 1.295 1.222 1.177
1.15
6ng/mL 0.609 0.648 0.603
0.687
3ng/mL 0.445 0.425 0.38
0.405
0ng/mL 0.028 0.045 0
0.062

Precision

We measured random samples of E1943Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.9 0.3 6
Intra-Assay 2 18 17.7 0.64 3.6
Intra-Assay 3 18 8.7 0.48 5.5

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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