Product overview
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 20000 pg/ml | 2.385 | 2.402 | 2.297 |
| 2.419 | |||
| 10000 pg/ml | 1.294 | 1.317 | 1.212 |
| 1.34 | |||
| 5000 pg/ml | 0.716 | 0.722 | 0.617 |
| 0.728 | |||
| 2500 pg/ml | 0.389 | 0.407 | 0.302 |
| 0.425 | |||
| 1250 pg/ml | 0.239 | 0.253 | 0.148 |
| 0.268 | |||
| 625 pg/ml | 0.161 | 0.163 | 0.058 |
| 0.165 | |||
| 312.5 pg/ml | 0.124 | 0.131 | 0.026 |
| 0.138 | |||
| 0 pg/ml | 0.108 | 0.105 | 0 |
| 0.102 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 1817 | 90.9 | 5 |
| Intra-assay | 2 | 20 | 4432 | 154 | 3.5 |
| Intra-assay | 3 | 20 | 12312 | 314 | 2.6 |
| Inter-assay | 1 | 20 | 1829 | 159 | 8.7 |
| Inter-assay | 2 | 20 | 4393 | 449 | 10.2 |
| Inter-assay | 3 | 20 | 12433 | 1167 | 9.4 |
Protocols
Customer reviews and Q&As
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