Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
15000 pg/ml | 2.357 | 2.27 | 2.182 |
2.184 | |||
7500 pg/ml | 1.203 | 1.148 | 1.06 |
1.094 | |||
3750 pg/ml | 0.618 | 0.599 | 0.511 |
0.58 | |||
1875 pg/ml | 0.348 | 0.343 | 0.255 |
0.338 | |||
937.5 pg/ml | 0.216 | 0.218 | 0.13 |
0.221 | |||
468.75 pg/ml | 0.162 | 0.155 | 0.067 |
0.148 | |||
234.375 pg/ml | 0.129 | 0.124 | 0.036 |
0.119 | |||
0 pg/ml | 0.089 | 0.088 | 0 |
0.088 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 1015 | 60.4 | 6 |
Intra-assay | 2 | 20 | 4228 | 242 | 5.7 |
Intra-assay | 3 | 20 | 10313 | 443 | 4.3 |
Inter-assay | 1 | 20 | 1008 | 55.6 | 5.5 |
Inter-assay | 2 | 20 | 4193 | 243 | 5.8 |
Inter-assay | 3 | 20 | 10340 | 493 | 4.8 |
Protocols
References
Customer reviews and Q&As