Product overview
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 6000 pg/ml | 2.407 | 2.523 | 2.392 |
| 2.639 | |||
| 3000 pg/ml | 1.466 | 1.474 | 1.343 |
| 1.482 | |||
| 1500 pg/ml | 0.865 | 0.919 | 0.788 |
| 0.973 | |||
| 750 pg/ml | 0.546 | 0.553 | 0.422 |
| 0.56 | |||
| 375 pg/ml | 0.341 | 0.357 | 0.226 |
| 0.373 | |||
| 187.5 pg/ml | 0.249 | 0.248 | 0.117 |
| 0.248 | |||
| 93.75 pg/ml | 0.189 | 0.186 | 0.055 |
| 0.183 | |||
| 0 pg/ml | 0.138 | 0.131 | 0 |
| 0.124 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 252 | 16.6 | 6.6 |
| Intra-assay | 2 | 20 | 811 | 37.8 | 4.7 |
| Intra-assay | 3 | 20 | 3528 | 107 | 3 |
| Inter-assay | 1 | 20 | 254 | 18.4 | 7.3 |
| Inter-assay | 2 | 20 | 814 | 42.2 | 5.2 |
| Inter-assay | 3 | 20 | 3544 | 159 | 4.5 |
Protocols
References
Customer reviews and Q&As
