Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
5000 pg/ml | 2.68 | 2.599 | 2.508 |
2.518 | |||
2500 pg/ml | 1.525 | 1.5 | 1.409 |
1.475 | |||
1250 pg/ml | 0.83 | 0.832 | 0.741 |
0.834 | |||
625 pg/ml | 0.432 | 0.447 | 0.356 |
0.461 | |||
312.5 pg/ml | 0.28 | 0.267 | 0.176 |
0.254 | |||
156.25 pg/ml | 0.181 | 0.177 | 0.086 |
0.173 | |||
78.125 pg/ml | 0.143 | 0.134 | 0.043 |
0.125 | |||
0 pg/ml | 0.094 | 0.091 | 0 |
0.088 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 266 | 14.5 | 5.5 |
Intra-assay | 2 | 20 | 1024 | 47.8 | 4.7 |
Intra-assay | 3 | 20 | 2925 | 63.4 | 2.2 |
Inter-assay | 1 | 20 | 265 | 20.5 | 7.7 |
Inter-assay | 2 | 20 | 1015 | 79 | 7.8 |
Inter-assay | 3 | 20 | 2941 | 188 | 6.4 |
Protocols
References
Customer reviews and Q&As