Product overview
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 5000 pg/ml | 1.451 | 1.462 | 1.331 |
| 1.473 | |||
| 2500 pg/ml | 0.875 | 0.879 | 0.748 |
| 0.883 | |||
| 1250 pg/ml | 0.497 | 0.499 | 0.368 |
| 0.501 | |||
| 625 pg/ml | 0.312 | 0.319 | 0.188 |
| 0.326 | |||
| 312.5 pg/ml | 0.2 | 0.204 | 0.073 |
| 0.208 | |||
| 156.25 pg/ml | 0.164 | 0.169 | 0.038 |
| 0.174 | |||
| 0 pg/ml | 0.134 | 0.131 | 0 |
| 0.128 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 502 | 23.6 | 4.7 |
| Intra-assay | 2 | 20 | 1091 | 47.8 | 4.4 |
| Intra-assay | 3 | 20 | 3410 | 92.1 | 2.7 |
| Inter-assay | 1 | 20 | 504 | 52.6 | 10.4 |
| Inter-assay | 2 | 20 | 1096 | 84.1 | 7.7 |
| Inter-assay | 3 | 20 | 3397 | 229 | 6.7 |
Protocols
References
Customer reviews and Q&As
