Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
4000 pg/ml | 2.426 | 2.515 | 2.388 |
2.603 | |||
2000 pg/ml | 1.217 | 1.224 | 1.097 |
1.23 | |||
1000 pg/ml | 0.631 | 0.623 | 0.496 |
0.615 | |||
500 pg/ml | 0.368 | 0.35 | 0.223 |
0.331 | |||
250 pg/ml | 0.23 | 0.232 | 0.105 |
0.233 | |||
125 pg/ml | 0.172 | 0.177 | 0.05 |
0.181 | |||
62.5 pg/ml | 0.151 | 0.143 | 0.016 |
0.134 | |||
0 pg/ml | 0.12 | 0.127 | 0 |
0.133 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 384 | 26.8 | 7 |
Intra-assay | 2 | 20 | 1202 | 42.6 | 3.5 |
Intra-assay | 3 | 20 | 3162 | 155 | 4.9 |
Inter-assay | 1 | 20 | 385 | 23.4 | 6.1 |
Inter-assay | 2 | 20 | 1204 | 59.9 | 5 |
Inter-assay | 3 | 20 | 3127 | 175 | 5.6 |
Protocols
References
Customer reviews and Q&As