Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
4000 pg/ml | 2.413 | 2.51 | 2.412 |
2.606 | |||
2000 pg/ml | 1.343 | 1.32 | 1.222 |
1.297 | |||
1000 pg/ml | 0.684 | 0.715 | 0.617 |
0.745 | |||
500 pg/ml | 0.37 | 0.38 | 0.282 |
0.39 | |||
250 pg/ml | 0.229 | 0.227 | 0.129 |
0.224 | |||
125 pg/ml | 0.15 | 0.152 | 0.054 |
0.154 | |||
62.5 pg/ml | 0.122 | 0.118 | 0.02 |
0.114 | |||
0 pg/ml | 0.103 | 0.098 | 0 |
0.093 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 165 | 11.7 | 7.1 |
Intra-assay | 2 | 20 | 716 | 29.9 | 4.2 |
Intra-assay | 3 | 20 | 2106 | 82.8 | 3.9 |
Inter-assay | 1 | 20 | 166 | 10.8 | 6.5 |
Inter-assay | 2 | 20 | 713 | 65.7 | 9.2 |
Inter-assay | 3 | 20 | 2089 | 167 | 8 |
Protocols
References
Customer reviews and Q&As