Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
3600 pg/ml | 3.082 | 3.001 | 2.897 |
2.92 | |||
1800 pg/ml | 1.707 | 1.791 | 1.687 |
1.876 | |||
900 pg/ml | 0.903 | 0.931 | 0.827 |
0.959 | |||
450 pg/ml | 0.516 | 0.508 | 0.404 |
0.5 | |||
225 pg/ml | 0.293 | 0.303 | 0.199 |
0.313 | |||
112.5 pg/ml | 0.19 | 0.198 | 0.094 |
0.206 | |||
56.25 pg/ml | 0.151 | 0.145 | 0.041 |
0.139 | |||
0 pg/ml | 0.102 | 0.104 | 0 |
0.106 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 298 | 9.9 | 3.3 |
Intra-assay | 2 | 20 | 932 | 31.1 | 3.3 |
Intra-assay | 3 | 20 | 1864 | 66.9 | 3.6 |
Inter-assay | 1 | 20 | 299 | 30.5 | 10.2 |
Inter-assay | 2 | 20 | 935 | 67.7 | 7.2 |
Inter-assay | 3 | 20 | 1885 | 135 | 7.2 |
Protocols
References
Customer reviews and Q&As