Product overview
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 3000 pg/ml | 2.358 | 2.34 | 2.257 |
| 2.322 | |||
| 1500 pg/ml | 1.169 | 1.201 | 1.118 |
| 1.233 | |||
| 750 pg/ml | 0.579 | 0.615 | 0.532 |
| 0.651 | |||
| 375 pg/ml | 0.324 | 0.334 | 0.251 |
| 0.344 | |||
| 187.5 pg/ml | 0.199 | 0.203 | 0.12 |
| 0.207 | |||
| 93.75 pg/ml | 0.129 | 0.135 | 0.052 |
| 0.141 | |||
| 46.875 pg/ml | 0.121 | 0.116 | 0.033 |
| 0.111 | |||
| 0 pg/ml | 0.085 | 0.083 | 0 |
| 0.081 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 201 | 6 | 3 |
| Intra-assay | 2 | 20 | 743 | 27.3 | 3.7 |
| Intra-assay | 3 | 20 | 1517 | 51.6 | 3.4 |
| Inter-assay | 1 | 20 | 202 | 13.1 | 6.5 |
| Inter-assay | 2 | 20 | 741 | 44.1 | 6 |
| Inter-assay | 3 | 20 | 1511 | 81.6 | 5.4 |
Protocols
References
Customer reviews and Q&As
