Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
3000 pg/ml | 2.852 | 2.782 | 2.647 |
2.711 | |||
1500 pg/ml | 1.667 | 1.595 | 1.46 |
1.522 | |||
750 pg/ml | 0.827 | 0.884 | 0.749 |
0.94 | |||
375 pg/ml | 0.514 | 0.495 | 0.36 |
0.475 | |||
187.5 pg/ml | 0.327 | 0.316 | 0.181 |
0.305 | |||
93.75 pg/ml | 0.221 | 0.223 | 0.088 |
0.225 | |||
46.875 pg/ml | 0.167 | 0.178 | 0.043 |
0.188 | |||
0 pg/ml | 0.128 | 0.135 | 0 |
0.142 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 378 | 15.6 | 4.1 |
Intra-assay | 2 | 20 | 804 | 33.6 | 4.2 |
Intra-assay | 3 | 20 | 1640 | 57.6 | 3.5 |
Inter-assay | 1 | 20 | 382 | 28.6 | 7.5 |
Inter-assay | 2 | 20 | 811 | 49.4 | 6.1 |
Inter-assay | 3 | 20 | 1637 | 133 | 8.2 |
Protocols
References
Customer reviews and Q&As