Product overview
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2800 pg/ml | 2.17 | 2.196 | 2.11 |
| 2.221 | |||
| 1400 pg/ml | 1.186 | 1.205 | 1.119 |
| 1.223 | |||
| 700 pg/ml | 0.628 | 0.661 | 0.575 |
| 0.694 | |||
| 350 pg/ml | 0.398 | 0.378 | 0.292 |
| 0.357 | |||
| 175 pg/ml | 0.249 | 0.235 | 0.149 |
| 0.221 | |||
| 87.5 pg/ml | 0.154 | 0.162 | 0.076 |
| 0.169 | |||
| 43.75 pg/ml | 0.124 | 0.122 | 0.036 |
| 0.119 | |||
| 0 pg/ml | 0.088 | 0.086 | 0 |
| 0.084 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 285 | 12.3 | 4.3 |
| Intra-assay | 2 | 20 | 696 | 22.8 | 3.3 |
| Intra-assay | 3 | 20 | 2110 | 103 | 4.9 |
| Inter-assay | 1 | 20 | 287 | 28.8 | 10 |
| Inter-assay | 2 | 20 | 704 | 48.2 | 6.8 |
| Inter-assay | 3 | 20 | 2103 | 60.9 | 2.9 |
Protocols
References
Customer reviews and Q&As
