Product overview
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2500 pg/ml | 2.308 | 2.284 | 2.199 |
| 2.261 | |||
| 1250 pg/ml | 1.163 | 1.23 | 1.145 |
| 1.297 | |||
| 625 pg/ml | 0.683 | 0.681 | 0.596 |
| 0.679 | |||
| 312.5 pg/ml | 0.417 | 0.403 | 0.318 |
| 0.389 | |||
| 156.25 pg/ml | 0.239 | 0.223 | 0.138 |
| 0.208 | |||
| 78.125 pg/ml | 0.137 | 0.146 | 0.061 |
| 0.155 | |||
| 39.0625 pg/ml | 0.114 | 0.117 | 0.032 |
| 0.12 | |||
| 0 pg/ml | 0.091 | 0.085 | 0 |
| 0.08 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 234 | 20.4 | 8.7 |
| Intra-assay | 2 | 20 | 586 | 43.5 | 7.4 |
| Intra-assay | 3 | 20 | 1332 | 108 | 8.1 |
| Inter-assay | 1 | 20 | 234 | 17 | 7.3 |
| Inter-assay | 2 | 20 | 587 | 46.5 | 7.9 |
| Inter-assay | 3 | 20 | 1340 | 90 | 6.7 |
Protocols
References
Customer reviews and Q&As
