Product overview
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2200 pg/ml | 2.124 | 2.192 | 2.078 |
| 2.26 | |||
| 1100 pg/ml | 1.195 | 1.244 | 1.13 |
| 1.292 | |||
| 550 pg/ml | 0.655 | 0.685 | 0.571 |
| 0.715 | |||
| 275 pg/ml | 0.424 | 0.403 | 0.289 |
| 0.381 | |||
| 137.5 pg/ml | 0.245 | 0.262 | 0.148 |
| 0.279 | |||
| 68.75 pg/ml | 0.177 | 0.178 | 0.064 |
| 0.179 | |||
| 34.375 pg/ml | 0.149 | 0.15 | 0.036 |
| 0.15 | |||
| 0 pg/ml | 0.108 | 0.114 | 0 |
| 0.119 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 148 | 12.9 | 8.7 |
| Intra-assay | 2 | 20 | 436 | 32.8 | 7.5 |
| Intra-assay | 3 | 20 | 1557 | 128 | 8.2 |
| Inter-assay | 1 | 20 | 149 | 10.9 | 7.3 |
| Inter-assay | 2 | 20 | 438 | 35 | 8 |
| Inter-assay | 3 | 20 | 1562 | 106 | 6.8 |
Protocols
References
Customer reviews and Q&As
