Product overview
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2000 pg/ml | 1.524 | 1.518 | 1.405 |
| 1.512 | |||
| 1000 pg/ml | 0.934 | 0.967 | 0.854 |
| 1 | |||
| 500 pg/ml | 0.535 | 0.567 | 0.454 |
| 0.599 | |||
| 250 pg/ml | 0.349 | 0.351 | 0.238 |
| 0.353 | |||
| 125 pg/ml | 0.238 | 0.237 | 0.124 |
| 0.236 | |||
| 62.5 pg/ml | 0.166 | 0.176 | 0.063 |
| 0.186 | |||
| 31.25 pg/ml | 0.145 | 0.146 | 0.033 |
| 0.148 | |||
| 0 pg/ml | 0.111 | 0.113 | 0 |
| 0.115 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 236 | 12.4 | 5.3 |
| Intra-assay | 2 | 20 | 525 | 16.9 | 3.2 |
| Intra-assay | 3 | 20 | 1314 | 30.2 | 2.3 |
| Inter-assay | 1 | 20 | 239 | 12.8 | 5.4 |
| Inter-assay | 2 | 20 | 530 | 29.3 | 5.5 |
| Inter-assay | 3 | 20 | 1317 | 65.1 | 4.9 |
Protocols
References
Customer reviews and Q&As
