Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
2000 pg/ml | 2.584 | 2.709 | 2.575 |
2.833 | |||
1000 pg/ml | 1.37 | 1.43 | 1.296 |
1.489 | |||
500 pg/ml | 0.825 | 0.772 | 0.638 |
0.719 | |||
250 pg/ml | 0.428 | 0.447 | 0.313 |
0.466 | |||
125 pg/ml | 0.293 | 0.291 | 0.157 |
0.289 | |||
62.5 pg/ml | 0.214 | 0.21 | 0.076 |
0.206 | |||
31.25 pg/ml | 0.168 | 0.169 | 0.035 |
0.17 | |||
0 pg/ml | 0.133 | 0.134 | 0 |
0.135 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 197 | 9.4 | 4.7 |
Intra-assay | 2 | 20 | 506 | 25.2 | 5 |
Intra-assay | 3 | 20 | 1033 | 46.2 | 4.5 |
Inter-assay | 1 | 20 | 196 | 16.3 | 8.3 |
Inter-assay | 2 | 20 | 508 | 44.1 | 8.7 |
Inter-assay | 3 | 20 | 1036 | 77.5 | 7.5 |
Protocols
References
Customer reviews and Q&As