Product overview
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600 pg/ml | 2.583 | 2.483 | 2.378 |
| 2.382 | |||
| 800 pg/ml | 1.513 | 1.474 | 1.369 |
| 1.435 | |||
| 400 pg/ml | 0.841 | 0.829 | 0.724 |
| 0.816 | |||
| 200 pg/ml | 0.473 | 0.467 | 0.362 |
| 0.461 | |||
| 100 pg/ml | 0.297 | 0.28 | 0.175 |
| 0.263 | |||
| 50 pg/ml | 0.193 | 0.186 | 0.081 |
| 0.178 | |||
| 25 pg/ml | 0.16 | 0.154 | 0.049 |
| 0.148 | |||
| 0 pg/ml | 0.104 | 0.105 | 0 |
| 0.106 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 118 | 6.2 | 5.3 |
| Intra-assay | 2 | 20 | 303 | 10.1 | 3.3 |
| Intra-assay | 3 | 20 | 977 | 22.8 | 2.3 |
| Inter-assay | 1 | 20 | 117 | 6.5 | 5.5 |
| Inter-assay | 2 | 20 | 305 | 17 | 5.6 |
| Inter-assay | 3 | 20 | 974 | 47.4 | 4.9 |
Protocols
References
Customer reviews and Q&As
