Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1500 pg/ml | 1.658 | 1.651 | 1.549 |
1.643 | |||
750 pg/ml | 1.02 | 0.96 | 0.858 |
0.899 | |||
375 pg/ml | 0.51 | 0.535 | 0.433 |
0.559 | |||
187.5 pg/ml | 0.332 | 0.311 | 0.209 |
0.289 | |||
93.75 pg/ml | 0.2 | 0.202 | 0.1 |
0.203 | |||
46.875 pg/ml | 0.147 | 0.156 | 0.054 |
0.165 | |||
23.4375 pg/ml | 0.129 | 0.132 | 0.03 |
0.134 | |||
0 pg/ml | 0.101 | 0.102 | 0 |
0.102 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 92.6 | 5.4 | 5.8 |
Intra-assay | 2 | 20 | 257 | 7.2 | 2.8 |
Intra-assay | 3 | 20 | 1118 | 24.2 | 2.2 |
Inter-assay | 1 | 20 | 93.1 | 6 | 6.4 |
Inter-assay | 2 | 20 | 256 | 10.5 | 4.1 |
Inter-assay | 3 | 20 | 1127 | 38.2 | 3.4 |
Protocols
References
Customer reviews and Q&As