Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1200 pg/ml | 2.48 | 2.439 | 2.322 |
2.399 | |||
600 pg/ml | 1.384 | 1.354 | 1.237 |
1.325 | |||
300 pg/ml | 0.768 | 0.717 | 0.6 |
0.667 | |||
150 pg/ml | 0.469 | 0.444 | 0.327 |
0.42 | |||
75 pg/ml | 0.293 | 0.275 | 0.158 |
0.257 | |||
37.5 pg/ml | 0.199 | 0.201 | 0.084 |
0.204 | |||
18.75 pg/ml | 0.165 | 0.16 | 0.043 |
0.156 | |||
0 pg/ml | 0.119 | 0.117 | 0 |
0.116 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 60.9 | 5.6 | 9.2 |
Intra-assay | 2 | 20 | 190 | 8.3 | 4.4 |
Intra-assay | 3 | 20 | 827 | 35.4 | 4.3 |
Inter-assay | 1 | 20 | 61.3 | 3.7 | 6 |
Inter-assay | 2 | 20 | 191 | 8.6 | 4.5 |
Inter-assay | 3 | 20 | 831 | 39.5 | 4.8 |
Protocols
References
Customer reviews and Q&As