Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
800 pg/ml | 2.341 | 2.406 | 2.322 |
2.47 | |||
400 pg/ml | 1.052 | 1.073 | 0.989 |
1.094 | |||
200 pg/ml | 0.589 | 0.596 | 0.512 |
0.602 | |||
100 pg/ml | 0.32 | 0.339 | 0.255 |
0.357 | |||
50 pg/ml | 0.215 | 0.219 | 0.135 |
0.223 | |||
25 pg/ml | 0.147 | 0.152 | 0.068 |
0.157 | |||
12.5 pg/ml | 0.125 | 0.118 | 0.034 |
0.11 | |||
0 pg/ml | 0.082 | 0.084 | 0 |
0.085 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 55.2 | 1.8 | 3.2 |
Intra-assay | 2 | 20 | 135 | 4.9 | 3.6 |
Intra-assay | 3 | 20 | 421 | 14.5 | 3.5 |
Inter-assay | 1 | 20 | 55 | 3.5 | 6.4 |
Inter-assay | 2 | 20 | 136 | 8.3 | 6.1 |
Inter-assay | 3 | 20 | 420 | 22.7 | 5.4 |
Protocols
References
Customer reviews and Q&As