Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
800 pg/ml | 1.913 | 1.843 | 1.715 |
1.773 | |||
400 pg/ml | 1.217 | 1.229 | 1.101 |
1.241 | |||
200 pg/ml | 0.753 | 0.792 | 0.664 |
0.831 | |||
100 pg/ml | 0.455 | 0.487 | 0.359 |
0.519 | |||
50 pg/ml | 0.293 | 0.312 | 0.184 |
0.331 | |||
25 pg/ml | 0.23 | 0.22 | 0.092 |
0.21 | |||
12.5 pg/ml | 0.165 | 0.169 | 0.041 |
0.173 | |||
0 pg/ml | 0.133 | 0.128 | 0 |
0.123 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 43 | 2.8 | 6.5 |
Intra-assay | 2 | 20 | 116 | 7.6 | 6.5 |
Intra-assay | 3 | 20 | 554 | 27.8 | 5 |
Inter-assay | 1 | 20 | 42.7 | 3.2 | 7.5 |
Inter-assay | 2 | 20 | 116 | 7.4 | 6.3 |
Inter-assay | 3 | 20 | 557 | 40.9 | 7.3 |
Protocols
References
Customer reviews and Q&As