Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
600 pg/ml | 1.922 | 1.874 | 1.745 |
1.826 | |||
300 pg/ml | 1.196 | 1.257 | 1.128 |
1.318 | |||
150 pg/ml | 0.737 | 0.769 | 0.64 |
0.801 | |||
75 pg/ml | 0.47 | 0.472 | 0.343 |
0.474 | |||
37.5 pg/ml | 0.3 | 0.303 | 0.174 |
0.306 | |||
18.75 pg/ml | 0.222 | 0.21 | 0.081 |
0.198 | |||
9.375 pg/ml | 0.174 | 0.169 | 0.04 |
0.163 | |||
0 pg/ml | 0.12 | 0.129 | 0 |
0.138 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 61.1 | 3 | 4.9 |
Intra-assay | 2 | 20 | 211 | 10.2 | 4.8 |
Intra-assay | 3 | 20 | 439 | 22 | 5 |
Inter-assay | 1 | 20 | 61.4 | 5.3 | 8.6 |
Inter-assay | 2 | 20 | 210 | 15.1 | 7.2 |
Inter-assay | 3 | 20 | 444 | 25.2 | 5.7 |
Protocols
References
Customer reviews and Q&As