Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
600 pg/ml | 2.251 | 2.26 | 2.165 |
2.27 | |||
300 pg/ml | 1.249 | 1.188 | 1.093 |
1.128 | |||
150 pg/ml | 0.658 | 0.663 | 0.568 |
0.669 | |||
75 pg/ml | 0.369 | 0.377 | 0.282 |
0.386 | |||
37.5 pg/ml | 0.25 | 0.238 | 0.143 |
0.227 | |||
18.75 pg/ml | 0.167 | 0.168 | 0.073 |
0.17 | |||
9.375 pg/ml | 0.124 | 0.123 | 0.028 |
0.122 | |||
0 pg/ml | 0.095 | 0.095 | 0 |
0.096 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 35.4 | 3.2 | 9 |
Intra-assay | 2 | 20 | 148 | 5.3 | 3.6 |
Intra-assay | 3 | 20 | 447 | 21.7 | 4.9 |
Inter-assay | 1 | 20 | 35.7 | 1.1 | 3.2 |
Inter-assay | 2 | 20 | 150 | 13.3 | 8.9 |
Inter-assay | 3 | 20 | 449 | 20.8 | 4.6 |
Protocols
References
Customer reviews and Q&As