Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
600 pg/ml | 2.78 | 2.859 | 2.764 |
2.937 | |||
300 pg/ml | 1.513 | 1.481 | 1.386 |
1.449 | |||
150 pg/ml | 0.769 | 0.822 | 0.727 |
0.874 | |||
75 pg/ml | 0.507 | 0.486 | 0.391 |
0.465 | |||
37.5 pg/ml | 0.304 | 0.29 | 0.195 |
0.276 | |||
18.75 pg/ml | 0.181 | 0.193 | 0.098 |
0.204 | |||
9.375 pg/ml | 0.142 | 0.14 | 0.045 |
0.137 | |||
0 pg/ml | 0.097 | 0.095 | 0 |
0.093 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 87.5 | 5.2 | 6 |
Intra-assay | 2 | 20 | 195 | 8.9 | 4.6 |
Intra-assay | 3 | 20 | 463 | 20.2 | 4.4 |
Inter-assay | 1 | 20 | 88.4 | 8.8 | 9.9 |
Inter-assay | 2 | 20 | 195 | 14.1 | 7.3 |
Inter-assay | 3 | 20 | 459 | 28.1 | 6.1 |
Protocols
References
Customer reviews and Q&As