Product overview
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 400 pg/ml | 2.833 | 2.737 | 2.618 |
| 2.641 | |||
| 200 pg/ml | 1.535 | 1.577 | 1.458 |
| 1.619 | |||
| 100 pg/ml | 0.753 | 0.758 | 0.639 |
| 0.763 | |||
| 50 pg/ml | 0.445 | 0.417 | 0.298 |
| 0.39 | |||
| 25 pg/ml | 0.303 | 0.284 | 0.165 |
| 0.266 | |||
| 12.5 pg/ml | 0.207 | 0.2 | 0.081 |
| 0.194 | |||
| 6.25 pg/ml | 0.169 | 0.159 | 0.04 |
| 0.149 | |||
| 0 pg/ml | 0.114 | 0.119 | 0 |
| 0.125 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 18 | 1.1 | 5.9 |
| Intra-assay | 2 | 20 | 48.7 | 2.8 | 5.8 |
| Intra-assay | 3 | 20 | 222 | 15.5 | 7 |
| Inter-assay | 1 | 20 | 18.1 | 1.4 | 7.8 |
| Inter-assay | 2 | 20 | 48.5 | 4.8 | 10 |
| Inter-assay | 3 | 20 | 225 | 14.6 | 6.5 |
Protocols
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