Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
350 pg/ml | 2.418 | 2.343 | 2.229 |
2.268 | |||
175 pg/ml | 1.339 | 1.351 | 1.237 |
1.363 | |||
87.5 pg/ml | 0.738 | 0.72 | 0.606 |
0.702 | |||
43.75 pg/ml | 0.423 | 0.421 | 0.307 |
0.419 | |||
21.875 pg/ml | 0.287 | 0.275 | 0.161 |
0.263 | |||
10.9375 pg/ml | 0.209 | 0.205 | 0.091 |
0.201 | |||
5.46875 pg/ml | 0.136 | 0.146 | 0.032 |
0.156 | |||
0 pg/ml | 0.111 | 0.114 | 0 |
0.117 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 39.5 | 1.7 | 4.4 |
Intra-assay | 2 | 20 | 110 | 3.5 | 3.2 |
Intra-assay | 3 | 20 | 224 | 6.7 | 3 |
Inter-assay | 1 | 20 | 39.6 | 3.1 | 7.8 |
Inter-assay | 2 | 20 | 110 | 8.5 | 7.7 |
Inter-assay | 3 | 20 | 226 | 12.4 | 5.5 |
Protocols
References
Customer reviews and Q&As