Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
150 pg/ml | 2.718 | 2.603 | 2.494 |
2.487 | |||
75 pg/ml | 1.464 | 1.533 | 1.424 |
1.601 | |||
37.5 pg/ml | 0.831 | 0.832 | 0.723 |
0.832 | |||
18.75 pg/ml | 0.479 | 0.458 | 0.349 |
0.436 | |||
9.375 pg/ml | 0.294 | 0.279 | 0.17 |
0.263 | |||
4.6875 pg/ml | 0.188 | 0.198 | 0.089 |
0.207 | |||
2.34375 pg/ml | 0.143 | 0.142 | 0.033 |
0.14 | |||
0 pg/ml | 0.103 | 0.109 | 0 |
0.114 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 5.1 | 0.16 | 3.2 |
Intra-assay | 2 | 20 | 17.5 | 0.61 | 3.5 |
Intra-assay | 3 | 20 | 83.5 | 2.9 | 3.4 |
Inter-assay | 1 | 20 | 5.1 | 0.33 | 6.4 |
Inter-assay | 2 | 20 | 17.5 | 1 | 6 |
Inter-assay | 3 | 20 | 83.8 | 4.5 | 5.4 |
Protocols
References
Customer reviews and Q&As