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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1425Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200nmol/L	2.032	2.167	2.122
	2.302
600nmol/L	1.325	1.376	1.331
	1.426
300nmol/L	0.603	0.683	0.638
	0.764
150nmol/L	0.419	0.391	0.346
	0.364
75nmol/L	0.184	0.17	0.125
	0.156
0nmol/L	0.05	0.045	0
	0.04

We measured random samples of E1425Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	148.8	5.6	3.8
Intra-assay	2	18	759.7	26.5	3.5
Intra-assay	3	18	529.9	24.5	4.6

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