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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1978Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
400ng/L	2.215	2.155	2.074
	2.095
200ng/L	1.208	1.171	1.09
	1.134
100ng/L	0.605	0.621	0.539
	0.636
50ng/L	0.307	0.324	0.242
	0.341
25ng/L	0.162	0.167	0.085
	0.172
0ng/L	0.076	0.081	0
	0.087

We measured random samples of E1978Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	211.7	8.7	4.1
Intra-assay	2	18	231.8	6.5	2.8
Intra-assay	3	18	108.1	4.5	4.2

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