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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0866Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.391	2.285	2.238
	2.178
60ng/ml	1.209	1.285	1.239
	1.361
30ng/ml	0.836	0.839	0.792
	0.841
15ng/ml	0.523	0.533	0.487
	0.544
7.5ng/ml	0.274	0.299	0.252
	0.324
0ng/ml	0.052	0.047	0
	0.041

We measured random samples of E0866Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	68.7	2.1	3.1
Intra-assay	2	18	49.6	2.1	4.2
Intra-assay	3	18	13.2	0.9	6.7

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