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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E7379Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	1.985	2.021	1.939
	2.058
60ng/ml	1.071	1.101	1.019
	1.131
30ng/ml	0.552	0.542	0.46
	0.532
15ng/ml	0.317	0.324	0.242
	0.33
7.5ng/ml	0.16	0.169	0.087
	0.179
0ng/ml	0.086	0.082	0
	0.077

We measured random samples of E7379Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	106.4	3.2	3
Intra-assay	2	18	58.9	2.9	5
Intra-assay	3	18	31.4	1.7	5.5

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