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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1911Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/ml	2.297	2.345	2.286
	2.393
120ng/ml	1.302	1.327	1.268
	1.352
60ng/ml	0.878	0.779	0.72
	0.68
30ng/ml	0.456	0.463	0.404
	0.469
15ng/ml	0.257	0.289	0.231
	0.322
0ng/ml	0.054	0.059	0
	0.064

We measured random samples of E1911Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	140	4.2	3
Intra-assay	2	18	100.9	6.1	6.1
Intra-assay	3	18	27.2	1.8	6.8

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