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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E4010Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/ml	2.056	2.054	2.021
	2.052
160ng/ml	1.465	1.432	1.399
	1.4
80ng/ml	0.731	0.753	0.72
	0.775
40ng/ml	0.44	0.437	0.404
	0.434
20ng/ml	0.197	0.156	0.123
	0.116
0ng/ml	0.033	0.033	0
	0.033

We measured random samples of E4010Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	204.4	10.1	5
Intra-assay	2	18	101.4	5.7	5.6
Intra-assay	3	18	57	2.5	4.4

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