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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0280Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	1.995	1.962	1.879
	1.929
3.2ng/ml	1.076	1.039	0.955
	1.002
1.6ng/ml	0.605	0.584	0.501
	0.564
0.8ng/ml	0.287	0.271	0.188
	0.255
0.4ng/ml	0.161	0.15	0.067
	0.139
0ng/ml	0.08	0.083	0
	0.087

We measured random samples of E0280Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	1.2	0.1	7
Intra-assay	2	18	2.2	0.1	4.4
Intra-assay	3	18	0.8	0.1	6.3

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